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In the alkaline stressed plants, the soluble protein content was dramatically reduced by 17.64 and 28.87% in 100 and 150 mM Na 2 CO 3 treatments, respectively, in comparison with that of 0 mM Na 2 CO 3 + 0 M JA untreated control plants

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N-acetylcysteine (NAC) provides cysteine, the rate-limiting amino acid in glutathione production

The samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose (NC) membranes (GE Healthcare)