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doi: 10.1016/j.bioadv.2022.213002 239 KimYWestGARayGKesslerSPPetreyACFiocchiCet al

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10 ul of each drug solution was added to 90 ul of the rat blood serum samples and at specific time intervals the researchers terminated metabolism by adding 1 ml of ACN and acetic acid in order to precipitate the proteins which were then removed after overnight refrigeration followed by centrifugation

Also consider a folate supplement (680 to 1,360 mcg DFE, or 400 to 800 mcg folic acid), particularly if you're a woman in your childbearing years