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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C

Safety Profile and Potential Side Effects Reported Adverse Effects Animal studies report minimal toxicity even at high doses

In aged mice, FOXO4-DRI treatment restored fur density, improved renal function, and increased physical activity levels, demonstrating functional rejuvenation through targeted senescent cell clearance

Once opened, store the vial at room temperature and access only with sterile needles using aseptic technique