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Since to our knowledge no systematic benchmark set for pHsolubility curves exists, we instead investigated three specific case studies for molecules with well-documented pH-dependent solubility
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Avoid freeze-thaw cycles and direct light exposure
Samples were desalted for 1 min and eluted onto the mass spectrometer using a 585% acetonitrile gradient over 2.5 min at a flow rate of 0.4 ml min 1
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