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Tyramide Signal Amplification for Immunofluorescent Enhancement
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The M1 phenotype of microglia is responsible for synthesizing and releasing various cytokines, including prostaglandin E2 (PGE2), CRP, and TNF-alpha, into the bloodstream (Attwells et al., 2020)
Reusing needles increases infection risk and dulls the tip, making injections more painful
Sheath and auxiliary gas flows were set to 60 and 11, respectively
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