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In protocol 2, we give 3.6 mM of NAC per hour for 10 hours beginning 2 hours after the 22 g APAP dose

The mobile phase consisted of a linear gradient system of phase A (60% acetonitrile/40% water solution containing 10 mM ammonium acetate and 0.1% formic acid) and phase B (isopropanol:acetonitrile, 9:1, v/v): 0 min 60% A, 2 min 57% A, 12 min 40% A, 12.1 min 25% A, 18 min 1% A, 19 min 1% A, and 20 min 40% A

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It plays a pivotal role in glucose metabolism and appetite regulation through multiple mechanisms: Enhances insulin secretion: GLP-1 stimulates pancreatic -cells to release insulin in a glucose-dependent manner Suppresses glucagon: Reduces hepatic glucose production Delays gastric emptying: Creates prolonged satiety Central nervous system effects: Acts on appetite centers in the brain to reduce hunger and food intake Protects pancreatic -cells: Promotes cell proliferation and inhibits apoptosis GLP-1 Receptor Agonists in Clinical Practice GLP-1 receptor agonists (GLP-1 RAs) are medications designed to mimic the action of endogenous GLP-1 but with enhanced pharmacokinetic properties