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Protein expression BL21(DE3) competent cells from NEB were transformed with the respective plasmid by heat shock following the provided standard procedure of the vendor

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a , Heat map of fold change (log 2 ) in unfolded protein response (UPR) target mRNAs from Slc33a1 -knockout KPK cells expressing a vector control compared to those expressing SLC33A1 cDNA

It belongs to a class of drugs that work by mimicking the functions of the naturally occurring hormone GLP-1, which helps regulate blood sugar levels

An insulin syringe is calibrated so that 100 units equals 1 mL, which means 50 units always equals half a milliliter