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Procedure The procedure for the GSSG application using the NWK-GSH01 assay is essentially the same as the standard method, with the following exceptions: The calibrators are made up using an MPA:Assay Buffer solution (2:1) instead of Assay Buffer

Timing differences can confound time-dependent outcomes in sensitive assays and analytical comparisons

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These damaged proteins can become joined together through a mechanism called crosslinking, which causes increased stiffness and loss of elasticity in several tissues throughout the body (Nawale 2006