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Tumor-associated macrophage-derived interleukin-23 interlinks kidney cancer glutamine addiction with immune evasion

N., and Thompson, C

Tissue lysate preparation The ipsilateral coronal brain tissue weighed between 75 and 100 mg, and it was homogenized in chilled condition using a micropestle with 1 ml of either extraction buffer (used for nitrite and malondialdehyde quantification) or radioimmunoprecipitation assay (RIPA) solution containing protease inhibitor

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Non-internalized bacteria were removed by discarding the supernatant and replacing it with fresh medium containing gentamicin sulfate (200 g/mL