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Four drops of fertilization medium (80 L/drop) were placed in a petri dish, covered with mineral oil (Sigma Chemical Co

doi: 10.1007/s00253-016-8032-4 23 KnezevicJStarchlCTmava BerishaAAmreinK

Contraindications Pregnant or breastfeeding women are usually advised to avoid glutathione injections due to a lack of studies in these populations

65 ) (Addgene, 52961) for CRISPRCas9 KO or into pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 66 (Addgene, 71236) for CRISPRi knockdown, as previously described 41

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