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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CASP8
Analyst 130:606625 Esau C, Davis S, Murray SF, Yu XX, Pandey SK, Pear M, Watts L, Booten SL, Graham M, McKay R, Subramaniam A, Propp S, Lollo BA, Freier S, Bennett CF, Bhanot S, Monia BP (2006) miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting
For optimal preservation of the longer TB-500 peptide bonds during reconstitution, Sterile Bacteriostatic Water (containing 0.9% benzyl alcohol) is the recommended laboratory solvent
We adopted an active comparator new user design where analysis was of new starters of each drug
Libraries were constructed from 250 ng of RNA using the NEBNext RNA Ultra II Directional Library Prep Kit (New England Biolabs)
