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Analysis of GPXs Retrieval of GPX genes/proteins Eight potential Arabidopsis GPX protein sequences, namely GPX1-8, obtained from the UniProtKB/Swiss-Prot database of NCBI were used as queries in Phytozome database to retrieve the very close homologs of GPX sequences in 18 plant species

To test for irreversibility and the type of inactivation occurring in Prx1 after the hydrogen peroxide pretreatment, 2 OD 600 units of cells were resuspended in 100 l SDSloading buffer containing either DMSO (unmodified), 10 mM TCEP (TCEP reduces disulfides and sulfenic acids only), or 10 mM mmPEG 24 (steady state), and boiled for 5 min at 96C

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