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This 1:1 ratio is not arbitraryit balances solution viscosity (too concentrated and the peptide may precipitate
Control rats (group 1) are fed with standard diet, while the intervention rats are fed with iodine-free diet for 12 weeks to induce hypothyroidism (groups 2-4) which is continued for four more weeks to allow screening of hypothyroid status and L-Thyroxine-treatment
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For IGF-1R/InsR HybridR activation, IGF-1R immunoprecipitates were probed with an antibody against the InsR specific pTyr 1334 residue (#44809G, Invitrogen/ThermoFisher) 52 , and total HybridR determined by immunoblotting with an Anti-InsR antibody (#3025)
Apr 2017;62(4):237-246
Rat beta-NGF DuoSet ELISA, R&D Systems, Cat# AF-556-NA) were analysed in the posterior cortex by enzyme-bound immunosorbent assay (ELISA) in a microplate photometer using commercial kits (Peprotech, So Paulo, Brazil)
