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The concentration of Mouse MPIF2 in the samples is then determined by comparing the OD of the samples to the standard curve

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To deplete gut microbiota, animals received a cocktail of 100 g/mL neomycin, 50 g/mL streptomycin, 100 g/mL ampicillin, 50 g/mL vancomycin, 100 g/mL metronidazole, 1 mg/mL bacitracin, 125 g/mL ciprofloxacin, and 100 g/mL ceftazidime (Sigma-Aldrich) in the drinking water (69)