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They replaced it

This evidence has inspired the ligandin model for vacuolar sequestration of anthocyanins ( 1 ), in which arGSTs are believed to protect the labile anthocyanidins ( 3 ) from degradation and/or guide them from their biosynthetic origin on the cytoplasmic surface of the endoplasmic reticulum to transporters located on the tonoplast membrane 18,19,31

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Post-incubation, the samples were centrifuged at 20,000 g for 30 min at 4 C to clarify the extracts

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