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Prepare assay buffers and consumables before opening the vial to reduce hold time

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Preparing reagents for calcium imaging For calcium imaging of the AWA neurons in response to diacetyl, we used two types of solutions: Stimulus and Buffer. Both solutions were composed of a CTM buffer supplemented with 10 mM Levamisole (Sigma, CAS Number: 16595805)

The barriers to doing these things are laughable. He considers the peptide craze even more troubling

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