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The peptide mixture was loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 m 2 cm, nano Viper C18) connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 m inner diameter, 3 m resin) in buffer A (0.1%formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology
Nature (2006) 444(7121):8607.10.1038/nature05485 6 NielsenSTJanumSKrogh-MadsenRSolomonTPMllerK
If you experience persistent or severe symptoms, consult with your healthcare provider
Warning signs to watch for include severe abdominal pain that radiates to the back, nausea that feels different from the usual GI side effects, and fever
Those who have failed oral medications often respond well to the injectable format
