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TB500: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Lys (or full TB4)
Prior to MS Analysis, the system was calibrated not only in mass dimension but also in ion mobility by using a spiked filter with reference calibrants (lock masses) with three Agilent ESI-L Tuning Mix ions, and the collision energy was decreased linearly from 45 eV at 1/K0 = 1.30 Vs cm 2 to 27 eV at 1/K0 = 0.85 Vs cm 2
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