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Lactate Dehydrogenase (LDH) Assay The relative amounts of LDH released into the media (conducted in triplicates for each group) were determined using CytoTox-ONE Homogeneous Membrane Integrity Assay kit Cat.#G7892 (Promega, USA) according to the manufacturers instructions

For microneedling steps, see the GHK-Cu microneedling guide

Ouwendijk et al., 2021)

Oxidative stress Multiple preclinical findings have demonstrated that LAN exposure induces oxidative stress responses, leading to the accumulation of reactive oxygen species (ROS), DNA damage, and altered expression of oxidative stress-related genes 60,61,62