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Repeated freeze-thaw cycles should be strictly limited to no more than three, as each cycle promotes peptide bond hydrolysis and aggregation that progressively reduces recoverable bioactive peptide content
How often can you get a B12 shot
The samples were diluted with formic acid to 50 l, filtered with a 0.22 m syringe filter (Millipore), and 5 l of the solution was injected into the LC-MS/MS
No aggregate purity or tested-content total is inferred
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