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This is why USP assigns a 28-day beyond-use date for multi-dose vials reconstituted with a preserved diluent like BWI [3]
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Cells were lysed in lysis buffer containing 10 mM Tris, pH 7.5, 100 M MgCl 2 , 5 mM -mercaptoethanol (-ME), 10 M GDP, 0.2 g/ml leupeptin, and 150 g/ml benzamidine
Then well get into the implications of the peptide surge
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