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Weigh Fmoc-Glu-OAllyl (40.9 g, 100.0 mmol) and HOBT (16.2 g, 110.0 mmol) and dissolve them in 150 mL of DMF, add DIC (20.4 mL, 120.0 mmol) under ice bath for activation for 5 min, and add the mixture to the reaction In the column, the reaction was carried out at room temperature for 2 hours

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Starting with small amounts and increasing gradually can help minimise these effects