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Bossart et al
Am J Physiol Regul Integr Comp Physiol (2007) 292(2):R897901.10.1152/ajpregu.00569.2006 203 KawakamiRSaitoYKishimotoIHaradaMKuwaharaKTakahashiNet alOverexpression of brain natriuretic peptide facilitates neutrophil infiltration and cardiac matrix metalloproteinase-9 expression after acute myocardial infarction
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For most users, that threshold sits between 0.25mg and 0.5mg daily during the loading phase
The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane
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