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Measuring a second messenger (e.g., cAMP or Ca 2+ ) supports pathway engagement, but proving that the pathway is causally responsible for the cellular response requires additional evidence such as receptor antagonists, pathway inhibitors, receptor knockdown or knockout, or orthogonal rescue experiments

You might be wondering why this is happening and whether it means something is wrong with your treatment

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Most side effects are temporary and manageable with proper titration and dietary adjustments