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This master pool was then inoculated into flasks with 50 mL of LB medium with 0.2% rhamnose at OD 600 0.025 ( ~ 75 CFU mutant -1 ) and grown at 37 C with 230 rpm shaking until early exponential phase (OD 600 0.15, about 56 h from frozen stocks)

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Treatment with roflumilast for 4 days significantly attenuated unfavorable changes in these parameters

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