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Refrigeration at 28 C is appropriate once the working timeline extends past a month
People with low B12 may experience: Persistent fatigue or low energy Weakness Brain fog, concentration issues, or memory changes Low mood or irritability Pins and needles, numbness, or nerve-type sensations Mouth ulcers or a sore tongue Paleness or shortness of breath (if anaemia is present) Some symptoms can become more serious if deficiency is prolonged, particularly neurological symptoms
avoid repeated freezethaw cycles.
A one or two week supply mixed fresh and kept cold remains stable long enough for use, though this approach requires more frequent preparation sessions
Bioinformatics 27 (21), 29572963
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