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Svane MS, Johannesen HH, Hansen AE, Martinussen C, Bojsen-Mller KN, Hansen ML, et al

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The concentration determines how much liquid contains those milligrams

A buffer consisting of 20 mM HEPES, 100 mM NaCl, pH 7.4, 10 mM MgCl 2 , 1 mM MnCl 2 , 100 M TCEP, 25 mU/mL apyrase (Sigma-Aldrich), 15 g/mL Nb35, protease inhibitor cocktail and 10% glycerol was used to resuspend the collected membranes