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The membrane was then washed three times with PBS-tween and incubated with horseradish peroxidase-conjugated secondary antibodies in 1% BSA in PBS-tween for 1 h at 22 C
JC-1 accumulates in mitochondria and reflects the membrane potential across the inner mitochondrial membrane
Am Surg 87(12):1934-1945, 2021
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