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In addition, human-interest senolytic dosing is typically pulsed/intermittent (a few consecutive days per cycle), so the continuous once-daily-for-16-weeks schedule shown here diverges from the typical senolytic paradigm and should be read as illustrative, not validated

Beyond that, degradation becomes measurable and progressive

After three further washes, peroxidase activity was visualized using diaminobenzidine chromogen (Dako), and slides were lightly counterstained with hematoxylin before dehydration and mounting in DePex (VWR International, Oslo, Norway)

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