Total RNA of HBE cells and lung tissues were extracted using a GenElute TM Mammalian Total RNA Miniprep Kit (Sigma, U.S.) according to the manufacturers protocol, and the concentration of total RNA was determined by measuring the absorbance at 260 nm using a Nanodrop 2000c spectrophotometer (Thermo Scientific, U.S.)
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Undissolved or precipitated magnesium simply cannot pass through your intestinal wall, no matter what the label says