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doi: 10.1126/science.aaf6284 65 Souto-CarneiroMMKlikaKDAbreuMTMeyerAPSaffrichRSandhoffRet al
Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment
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The troche is a square-shaped solid dosage form that is designed to dissolve slowly in the mouth, allowing for the medication to be absorbed through the oral mucosa for targeted local or systemic effects
First shipment did not come with syringes but online support was able to resolve quickly and syringes came within a few days
