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GLP-1R co-localisation with clathrin-coated pits (CCPs) with using TIRF microscopy INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were transfected with clathrin light chain (CLC)-GFP and plated onto poly-L-lysine-coated glass coverslips
Wrenn, F
The membrane was washed three times with TTBS for 5 min each and incubated with horseradish peroxidase conjugated goat anti-mouse IgG (Sigma) diluted (1:3000) in TTBS at 37C for 1 h
(Context for GH receptor binding site differences.) Manning MC, Chou DK, Murphy BM, Payne RW, Katayama DS
Curr Med Chem, 2006
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