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Immunoblot assays Cell lysates from HCC-1806, PC3 WT and PC3 GGT1 + cells were obtained by placing PBS-washed cell plates on ice and scraping with RIPA buffer (Thermo Scientific, 89900) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280)
Moradi Vastegani, S
TB-500 separate (typically 4-8x 5mg vials for 4-week protocol)
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