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Cells were maintained under standard conditions: cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin, and incubated at 37 C in a humidified environment with 5% CO
Su concentracin plasmtica es del orden de 200 ng/ml en el adulto joven (alrededor de 20 aos), pero desciende a aproximadamente 80 ng/ml hacia los 60 aos
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A scheme integrating all MTX-modulated metabolites into a single metabolic panel can be found in Supplementary Figure 4
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