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NAPQI-GST Formation by RLM Incubation RLM (1 mg/mL protein) and APAP (100 M) were incubated (37C and 500 rpm) in the presence of NADP + (500 M), and a NADPH-regenerating system: glucose 6-phosphate (10 mM), glucose-6-phosphate dehydrogenase (2 U/mL) and magnesium chloride (5 mM)